Fuente: WIPO "tomato" Disclosed herein are enucleated cells comprising an exogenous RNA encoding an antigen presenting cell (APC) activator or engineered to express an exogenous APC activator; and an exogenous RNA encoding a cancer antigen or engineered to express a cancer antigen. Disclosed are enucleated cells comprising an exogenous APC activator and a cancer antigen. Also disclosed are methods of treating subjects comprising administering to the subject one or more enucleated cells disclosed herein.