Fuente: WIPO "tomato" Disclosed herein are enucleated cells comprising an exogenous nucleic acid molecule encoding a. CD40L polypeptide or engineered to express an exogenous CD40L polypeptide. Disclosed are enucleated cells comprising exogenous CD40L polypeptide. Also disclosed are methods of treating subjects comprising administering to the subject one or more enucleated cells disclosed herein.