Sense-and-Response of Proteins, Peptides, and Small Molecules Using Ligand-Induced Dimerization Activating RNA Editing (LIDAR)

Fuente: WIPO "tomato"
The present disclosure provides a method for ligand induced ADAR (adenosine deaminase acting on RNA) mediated expression of an RNA coding sequence, the method comprising contacting a ligand with a cell associated LIDAR meditated expression system comprising: 1) an output RNA, 2) a stem loop binding protein and 3) an RNA editing protein; to express the RNA coding sequence. The present disclosure also provides compositions and kits for practicing the methods disclosed herein.