Fuente: WIPO "tomato" The present invention relates to the field of biotechnology. More specifically, the present invention relates to a mutant insecticidal protein Vip3 and its application. By performing point mutation on each original Vip3 family protein sequence in the present invention, a mutant insecticidal protein Vip3 with reduced plant cytotoxicity and high expression in plant cells is obtained, which has excellent insecticidal effects on various insect pests.