Fuente:
WIPO "tomato"
Provided is a method for isolating nucleic acids from a plant sample comprising
(a) preparing a lysed sample wherein preparing comprises
(i) lysing a plant sample by mechanically disrupting the plant sample in a lysis solution which comprises at least one chaotropic agent and one or more solid disrupting particles, and
(ii) optionally clearing the lysate;
(b) contacting the lysed sample with at least one protein precipitating agent and at least one inhibitor removing agent and providing a mixture;
(c) obtaining a liquid phase from the mixture; and
(d) isolating nucleic acids from the liquid phase.
Also provided is a kit for use in such method.