HIGH-THROUGHPUT METHOD FOR MITOCHONDRIA DNA ISOLATION FROM PLANT SEEDS

Fuente: WIPO "tomato"
The invention relates to methods of extracting mitochondrial DNA from whole seeds virtually free of nuclear DNA in a high-throughput environment. Methods also relate to the use of a solid phase nuclear DNA binder to selectively remove nuclear DNA from a sample solution comprising dry seed powder resuspended in an alkaline adsorption buffer and extracting mitochondrial DNA from a supernatant of the sample solution treated with the solid phase nuclear DNA binder. The solid phase nuclear DNA binder comprises Cerium oxide powder or Iron Oxide powder in particular embodiments.