Fuente: WIPO "tomato" The disclosure provides improved Cas-CLOVER systems for gene editing. In embodiments, the disclosure provides recombinant Clo051 endonucleases, or nuclease domains thereof, comprising one or more amino acid mutations (e.g. one or more amino acid substitutions at E101 and/or F44). The disclosure also provides fusion proteins, comprising: a DNA localization component, and any one of the Clo051 endonucleases disclosed herein or the nuclease domains thereof, and further provides methods of using the fusions proteins in gene editing, including introducing a double stranded break in a target nucleic acid.