Fuente: WIPO "tomato" Described herein are engineered proteins and methods of use of such proteins. A protein described herein may include an amino acid sequence having at least 70% sequence identity to SEQ ID NO:386 when optimally aligned thereto, wherein the amino acid sequence, when optimally aligned to SEQ ID NO:386, has an arginine residue at amino acid residue 14, 152, 156, 157, 159, 160, 165, 167, 168, 169, 251, 257, 260, 264, 272, 497, 653, 657, 660, 662, 1043, 1045, 1092, 1093, 1096, 1127, 1129, 1130, 1132, and/or 1133 corresponding to the amino acid residue position numbering of SEQ ID NO:386. Also described herein are complexes, compositions, and systems including a protein of the present invention, each of which may be used for modifying and/or editing a target nucleic acid.