Fuente: WIPO "tomato" The present disclosure provides dimeric deamination polyproteins comprising a first nucleotide deaminase, a second nucleotide deaminase, a first nuclease-active Cas-alpha 10 polypeptide, and a nuclease-active second Cas-alpha 10 polypeptide. Also disclosed are methods of modifying a double-stranded DNA target site and methods of multiplexed editing using a deamination polyprotein and guide polynucleotides, wherein a dimeric deamination polyprotein and the guide polynucleotide form a complex that recognizes and binds to a dsDNA target site.