COMPOSITIONS AND METHODS FOR ENHANCED TRANSLATION IN PLANTS

Fuente: WIPO "tomato"
In accordance with the purpose(s) of the disclosure, as embodied and broadly described herein, the disclosure, in one aspect, relates to methods and compositions for enhanced expression of a gene by enhanced translation of the gene, e.g., by operably linking a disclosed 5' UTR to the gene whose expression is to be enhanced. In a further aspect, the enhanced expression relates to enhanced expression MYB28, and thereby, enhancing the expression of genes regulated by MYB28. Disclosed are expression cassettes for enhancing translation of a gene comprising at least a portion of the 5' UTR of MYB28 operably linked to: (a) a gene to be expressed downstream of the 5' UTR of MYB28; (b) a 3' UTR downstream of the gene to be expressed; and (c) a promoter upstream of the 5' UTR of MYB28. Also disclosed are nucleic acid vectors comprising a disclosed expression cassette; recombinant plant cells capable of exhibiting enhanced translation of a gene, wherein said cell comprises a construct comprising at least a portion of the 5' UTR of MYB28 gene upstream of the gene to be expressed; and plants comprising a disclosed recombinant plant cell.