Fuente: WIPO "tomato" The present disclosure provide for methods of detecting Mayaro virus in a sample, assays for the detection of the Mayaro virus, and Mayaro virus-specific primers. An assay of the present disclosure can include Mayaro virus (MAYV)-specific primers, such as a MAYV-specific forward inner primer, a MAYV-specific backward inner primer, a MAYV-specific forward primer, and a MAYV-specific backward primer. A method provided in the present disclosure of detecting MAYV in a sample can include adding an amount of the sample to a reaction mixture and amplifying the RNA in the sample using reverse transcription loop-mediated isothermal amplification.