Fuente: PubMed "stone fruits" Virusdisease. 2026 Mar;37(1):75-86. doi: 10.1007/s13337-026-00957-x. Epub 2026 Apr 4.ABSTRACTApple chlorotic leafspot virus (ACLSV) and prunus necrotic ringspot virus (PNRSV) are among the most prevalent and commercially significant viruses infecting stone fruits. This study aimed to identify and characterize ACLSV and PNRSV infecting major stone fruit crops in Jammu & Kashmir. Extensive field surveys were conducted during the spring seasons of 2020-2022 across different stone fruit growing areas of Kashmir valley. Leaf samples exhibiting symptoms suggestive of virus infection such as shot holes, necrotic spots, chlorotic spots, severe rugose mosaic etc. were collected, along with the corresponding healthy leaf samples from the same plants. These samples were tested serologically using commercially available DAS-ELISA kits and on the basis of OD405 (optical density) values, plum samples exhibited the highest concentration of viral titer (1.986), while cherry samples had the lowest (1.002) for ACLSV and cherries had the highest PNRSV titer (0.486), while nectarine had the lowest (0.362). Further, molecular identification was done through RT-PCR assay using coat protein (CP) gene specific primers of ACLSV and PNRSV in order to re-verify ELISA results. Among the five major stone fruits, plum was found to be highly infected with ACLSV (45.0%) followed by cherry (37.5%), peach (32.5%), nectarine (27.5%), and apricot (22.5%). Overall, cherry showed the greatest PNRSV infection rate (64%), followed by plum (36%), nectarine (40%), peach (32.5%), and apricot (10%). As a result, this study confirmed stone fruit crops viz., peach, nectarine, plum, apricot and cherry from the temperate zone of J&K as natural hosts to ACLSV and PNRSV.PMID:42021945 | PMC:PMC13096282 | DOI:10.1007/s13337-026-00957-x