Fecha de publicación: 30/09/2022 Fuente: WIPO "hemp" The invention provides a probiotic composition capable of balancing intestinal flora and a preparation method and application thereof, and belongs to the technical field of probiotics. Cured shaddock peel and honey are mixed, bacillus coagulans TQ33 is inoculated for fermentation, then the mixture is mixed with a liquid culture medium containing dandelion extract, puerarin, a carbon source, a nitrogen source, vitamins and inorganic salt, and the probiotic composition is obtained. And inoculating lactobacillus acidophilus JYLA-191 and saccharomyces cerevisiae for fermentation, and uniformly mixing the obtained fermentation mixture with hemp seed oil and bat dung to obtain the probiotic composition for balancing intestinal flora. The composition prepared by the invention has a good effect of improving and balancing flora, is beneficial to inhibiting the reproduction of pathogenic bacteria, promoting the reproduction of beneficial bacteria and regulating the abundance of intestinal flora, and has a good regulating effect on metabolic diseases such as diabetes mellitus, hyperlipidemia, hyperglycemia, obesity and the like.