Fecha de publicación: 09/09/2022 Fuente: WIPO "hemp" The invention provides a method for cultivating all-female seedlings of hermaphrodite industrial cannabis sativa, and belongs to the technical field of vegetative propagation of plants. The cultivation method comprises the following steps that industrial hemp seedlings are subjected to short photoperiod cultivation, the illumination time is 8-10 h/day, and the industrial hemp seedlings are determined as female plants when pistils appear at the top ends of the plants; the female plants are topped, light supplementing culture is carried out, and the illumination time is 16-18 h/day; pinching is conducted when primary branches of the female plants grow to 20 cm, and when secondary branches grow to 20-25 cm, the primary branches serve as cutting materials, and all-female seedlings of industrial cannabis sativa are obtained through cutting. According to the method, all-female seedlings of the hermaphrodite industrial cannabis varieties are rapidly cultivated by adjusting the photoperiod, only 90-120 days are needed, the production technology of the industrial cannabis for floral leaves is greatly simplified, and labor investment is reduced.