Fecha de publicación: 26/07/2022 Fuente: WIPO "hemp" The invention discloses an industrial cannabis sativa germplasm resource preservation and breeding method. The industrial cannabis sativa germplasm resources which are uniform in size, complete, full, free of diseases and pests and mature in development are selected; the culture medium is seedling culture soil, before planting, a nutrition pot and the culture medium are disinfected with a 0.5% potassium permanganate solution, a culture pot and a hollow hard plastic irrigation pipe are prepared, the irrigation pipe is obliquely placed in the culture pot, a pipe opening of the irrigation pipe is higher than the upper edge of the culture pot, and the culture medium is placed in the culture pot. The invention provides an industrial hemp germplasm resource preservation and breeding method, which can safely and effectively preserve the diversity of industrial hemp single plant heredity, can provide abundant original materials for the cultivation of new varieties, can improve the predictability of breeding, can effectively preserve industrial hemp planting resources, and has the advantages of wide application prospect and wide application prospect. And material guarantee and technical support are provided for industrial cannabis sativa germplasm resource preservation and innovative utilization.