Fuente: WIPO "industrial hemp" The invention discloses a method for rapidly purifying and propagating industrial hemp by utilizing stem tip and stem segment tissue culture, relates to the field of tissue culture, and solves the problem of purifying and storing industrial hemp germplasm resources through a stem tip and stem segment tissue rapid propagation technology of medical, fiber and seed industrial hemp sterile seedlings or potted explants. And the progeny material with excellent germplasm characteristics can be rapidly purified and propagated. According to the method, a DKW culture medium containing TDZ is adopted for respectively carrying out differentiation culture on sterile stem tips and sterile stem explants, then rooting culture is carried out, and obtained tissue culture seedlings are domesticated and transplanted. According to the method, the selection specificity of industrial hemp varieties from different sources is not high, the breeding efficiency of medicinal, fiber and seed industrial hemp varieties is very high and can reach 70% or above, the effect of the medicinal industrial hemp varieties is particularly better, the breeding efficiency and rooting efficiency of stem tips and stem segments can reach 90-100%, the growth rate of the stem tips and the stem segments is higher, and the method is suitable for large-scale popularization and application. The industrial hemp breeding period is shorter.