Fuente: WIPO "hemp" The invention discloses a method for detecting cannabinoid in industrial hemp floral leaves and extracts thereof by high performance liquid chromatography. The method is characterized by comprising the following steps: (1) preparing floral leaf extraction solution: carrying out ultrasonic extraction on floral leaf powder by using a first extraction solvent, carrying out centrifugal separation, andfiltering to obtain floral leaf extracting solution; (2) preparing an extract sample: carrying out ultrasonic extraction on the floral leaf extracting solution by utilizing a second extracting solvent, and filtering to obtain an extract detection sample; (3) preparing a standard working solution: diluting a cannabinoid standard substance into the standard working solution by using methanol; and (4) high performance liquid chromatography determining: carrying out sample introduction detection on the extract detection sample and the standard working solution by using high performance liquid chromatography to obtain a standard curve, and calculating the cannabinoid component content of the extract detection sample according to the standard curve by using high performance liquid chromatography data processing software. The method has the advantages of short peak appearance time, short detection time, high detection efficiency and accurate and stable detection result, and is suitable for industrial detection.