A LIQUID MICROBIAL CONSORTIUM FOR BIOHYDROGEN GENERATION AND A PROCESS THEREOF

Fecha de publicación: 12/01/2023
Fuente: WIPO Rice
The present invention relates to a liquid microbial consortium for biohydrogen generation and a process thereof. The liquid microbial consortium comprises of Clostridium chromiireducens strain CTS0513, Clostridium chromiireducens strain STS0514, Clostridium chromiireducens strain XTS0511, Clostridium diolis strain STS0519, and Clostridium diolis strain XTS0513. The invention relates to a process for two-stage microbial degradation leading to direct production of recoverable biohydrogen with the help of microbial fermentation of lignocellulosic crop residues by disclosed consortium in Stage I, followed by generation of biomethane in Stage II from the digestate of Stage I. The disclosed consortium has higher growth rate and broad optimal growth range that ensures stability, reproducibility, and scalability. The improved biohydrogen production also eliminates the requirement of harsh pretreatment of substrates, sterilization of the substrates/ containers/equipment, need for repetitive dosing of disclosed consortium, and addition of acids/ alkali to maintain pH or inert gases/ reducing agents to maintain anaerobic conditions.