Fuente: Biomolecules - Revista científica (MDPI) Biomolecules, Vol. 16, Pages 758: Growth of Escherichia coli in Minimal Media Supplemented with N6-Methylated but Not N6,N6-Dimethylated Purines Is Supported by Adenosine Deaminase Add
Biomolecules doi: 10.3390/biom16060758
Authors:
Jaunius Urbonavičius
Augusta Ivaškė
Daiva Tauraitė

N6-methyladenine and N6,N6-dimethyladenine are the heterocyclic bases present in the RNA of eukaryotic and bacterial cells and play important regulatory roles. How the degradation of such modified nucleic acids, and the subsequent demethylation of modified heterocyclic bases, occurs in the bacterium Escherichia coli is not established. Here, we investigated the growth of adenine auxotroph strains in a minimal M9 medium supplemented with either N6-methyladenine or N6,N6-dimethyladenine. We found that N6-methyladenine supported the growth of ∆purH::Km but not that of the ∆purA::Km strain, whereas N6,N6-dimethyladenine did not support the growth of either adenine auxotroph. Similar experiments performed using structurally related 2-amino-N6-methylpurine and 2-amino-N6,N6-dimethylpurine bases—using ∆guaA::Km, ∆guaB::Km, and ∆purH::Km guanine auxotrophs—demonstrated that growth of only the ∆guaB::Km mutant was supported by 2-amino-N6-methylpurine but not by its dimethylated counterpart. We expressed and purified C-teminus 6xHis tagged E. coli adenine/adenosine deaminases AdeC and Add and tested their substrate specificity. We demonstrated that AdeC protein does not catalyse deamination of either N6-methyl- or N6,N6-dimethyladenine, whereas Add catalyses deamination of N6-methyl- but not that of N6,N6-dimethyladenosine. Based on our findings, biochemical pathways leading to the demodification and return into metabolism of N6-methyladenine and 2-amino-N6-methylpurine in E. coli are proposed.