Fecha de publicación: 15/12/2024 Fuente: Microorganisms - Revista científica (MDPI) Microorganisms, Vol. 12, Pages 2596: Release of Biopolymers from Saccharomyces cerevisiae Biomass Through Thermal and Non-Thermal Technologies
Microorganisms doi: 10.3390/microorganisms12122596
Authors:
Marianna Ciccone
Muhammad Rehan Khan
Junior Bernardo Molina Hernandez
Joel Armando Njieukam
Lorenzo Siroli
Davide Gottardi
Rosalba Lanciotti
Pietro Rocculi
Francesca Patrignani

Components of yeast cell walls, such as β-glucans and mannoproteins, show promise for developing sustainable biopolymers for food packaging. Efficient extraction, however, is challenging due to the complexity of the yeast cell wall. This study explored high-pressure homogenisation (HPH) and pulsed electric fields (PEFs), alone and with heat treatment (TT), on bakery yeast (BY) and brewery spent yeast (BSY) biomasses. In the treated samples we assessed carbohydrates, proteins, β-glucans, and mannoproteins and evaluated cell wall disruption microscopically. HPH caused complete cell disintegration, enhancing intracellular release, while PEF primarily permeabilised the membranes. Combined HPH and PEF treatments significantly increased cell wall stress, leading to partial disintegration. Notably, the β-glucans released reached 3.90 g/100 g dry matter in BY and 10.44 g/100 g dry matter in BSY, demonstrating significant extraction improvements. These findings highlight the potential of HPH and PEF for enhancing β-glucan recovery from yeast biomass, offering a promising route for sustainable biopolymer production for food packaging.