Fuente: Foods - Revista científica (MDPI) Foods, Vol. 15, Pages 829: A Fluorescent Aptasensor Based on Tb-MOFs and Its Application for the Detection of Pseudomonas aeruginosa in Foods
Foods doi: 10.3390/foods15050829
Authors:
Jinqiong Xu
Xinyu Yu
Jianbo Liu
Qinglin Sheng

Pseudomonas aeruginosa is an important waterborne pathogen that is harmful to food safety and human health. Therefore, the detection of Pseudomonas aeruginosa has become an essential item in food sampling. This article constructs a fluorescent aptamer sensor based on Tb-MOFs, using Tb-MOFs as the fluorescence signal source, colloidal gold (AuNPs) as the signal conversion switch, and aptamers as the target recognition element, to establish a quantitative detection method for Pseudomonas aeruginosa. The constructed sensor exhibits a good linear relationship within the concentration range of Pseudomonas aeruginosa from 1 to 106 CFU/mL, with a detection limit of 0.63 CFU/mL. Moreover, the sensor was also applied to the detection of Pseudomonas aeruginosa in actual samples of bottled drinking water and orange juice. The fluorescent aptasensor based on Tb-MOFs provides a rapid and sensitive new sensing method for the detection of Pseudomonas aeruginosa in food.