Fecha de publicación:
20/12/2024
Fuente: Molecules - Revista científica (MDPI)
Molecules, Vol. 29, Pages 6010: Kalanchoe crenata Andrews (Haw.) Improves Losartan’s Antihypertensive Activity
Molecules doi: 10.3390/molecules29246010
Authors:
Pedro de Padua G. Amatto
Juliana da Silva Coppede
Carla Renata Kitanishi
Giovana Graça Braga
Thaysa Carvalho de Faria
Elen Rizzi
Suzelei de Castro França
Fernanda Basso
Adriana Aparecida Lopes
Fábio Carmona
Silvia Helena Taleb Contini
Ana Maria Soares Pereira
Background: Cardiovascular diseases constitute one of the leading causes of morbidity and mortality worldwide. Herbal medicines represent viable alternatives to the synthetic drugs currently employed in the control of hypertension. This study aimed to isolate and identify the chemical markers of Kalanchoe crenata and to investigate the antihypertensive and anti-matrix metalloproteinase (MMP2) activities of an aqueous extract of the leaves. Methods: The main constituents of the aqueous extract of K. crenata were separated by ultra-performance liquid chromatography–mass spectrometry, and their presence was identified by NMR spectroscopy. Renovascular hypertension was induced in male Wistar rats using the two-kidney one-clip method (HTN groups), while control animals (Sham groups) were submitted to Sham surgery. Six groups of 10 animals each were treated daily for eight weeks as follows: Sham 1 (carrier), Sham 2 (K. crenata extract), HTN.1 (carrier), HTN.2 (K. crenata extract), HTN 3 (losartan), and HTN 4 (K. crenata extract with losartan). Results: The main compounds of the extract were patuletin 3-O-(4″-O-acetyl-α-L-rhamnopyranosyl)-7-O-(3‴-O-acetyl-α-L-rhamnopyranoside) (1), patuletin 3-O-α-L-rhamnopyranosyl-7-O-L-rhamnopyranoside (2), and trans-caffeoyl-malic acid (3), with compounds 1 and 2 being chemical markers of the species. Significant reductions (p < 0.05) in systolic blood pressure and MMP2 (72kDa isoform) activity were observed in the HTN 4 group. Conclusions: The association of K. crenata extract and losartan presented in vivo effects against hypertension.
