Fecha de publicación: 09/10/2008
Fuente: WIPO Agriculture Portada
The phylogeny of twelve Campylobacter species was studied based on partial (1020- bp) gyrB gene (DNA topoisomerase beta-subunit gene) sequences. The topology of the resulting phylogenic neighbor-joining tree based on the gyrB gene provides a better resolution than the 16S rDNA gene for Campylobacter species with interspecies sequence similarities ranging from 58.3 to 89.2%. A universal primer set was developed and used for PCR restriction fragment length polymorphism (PCR-RFLP) of 19 strains representing twelve Campylobacter species. PCR assays were developed using species-specific primer sets for amplification of regions of the gyrB gene specific for each Campylobacter species. PCR-RFLP analysis, and PCR using the species- specific primer sets based on the gyrB gene provide valuable tools for rapid detection and unambiguous identification of the majority of Campylobacter species.