Fuente: Journal of applied polymer Lugar: RESEARCH ARTICLE Schematic illustration of the preparation route for the electrospun PCL/NP/AA system, showing AA extract preparation, formation of AA associated β-glucan nanoparticles, incorporation of the NP/AA fraction into the PCL solution, and electrospinning of the composite fiber mat.

ABSTRACT
Persistent and hard to heal wounds require dressings that do more than cover the injured site; they must also support cell contact and enable local presentation of bioactive compounds. In this study, electrospun polycaprolactone (PCL) mats incorporating Artemisia absinthium (AA) associated β-glucan nanoparticles were developed as a wound relevant material system. The formulation was evaluated in terms of nanoparticle size, fiber morphology, surface wettability, chemical signatures, and comparative phytochemical profiling. Dynamic light scattering showed that filtered β-glucan nanoparticles had an average diameter of about 40 nm, while scanning electron microscopy confirmed bead free fiber formation in pristine PCL and PCL/NP fibers. Water contact angle decreased from 143.6° for neat PCL to 71.5° for PCL/NP/AA, indicating a marked increase in wettability. FTIR analysis supported the presence of β-glucan and extract associated chemical signatures in the fiber system. HPLC based comparative profiling further showed that several major AA phytochemical markers remained detectable after nanoparticle preparation and fiber fabrication, although encapsulation efficiency and time dependent release kinetics were not determined. In preliminary biological assessment, hemolysis decreased from 10.8% to 2.5% under the tested conditions; scratch assay findings showed improved cell migration in the PCL/NP/AA group relative to comparator groups; and nonstandard short contact antibacterial testing showed strain dependent reductions in relative bacterial viability. In silico analysis provided exploratory support for interactions between selected AA associated phytochemicals and wound related inflammatory targets. Taken together, these findings support the potential of the composite as a wound relevant electrospun material with improved wettability, detectable extract associated phytochemical signatures, and preliminary biological readouts relevant to wound related applications.