Fuente: Polymers Polymers, Vol. 17, Pages 3284: The Temporal Extracellular Proteomics Analysis Reveals the Expression Patterns of Functional Enzymes Involved in Ramie Degumming by Dickeya dadantii Strain DCE-01
Polymers doi: 10.3390/polym17243284
Authors:
Yuqin Hu
Mingqiang Gao
Xiang Zhou
Lifeng Cheng
Guoguo Xi
Si Tan
Wei Zhou
Zishu Chen
Zhenghong Peng
An Wang
Shengwen Duan
Qi Yang

Microbial degumming offers an environmentally sustainable route for the extraction of natural ramie fibers. However, there are currently no genetically engineered bacteria suitable for large-scale industrial production. In this study, Dickeya dadantii strain DCE-01, a dominant ramie-degumming bacterium, was systematically investigated using time-resolved quantitative proteomics. This research revealed a temporally coordinated, multi-enzyme catalytic mechanism, in which pectate lyases were highly expressed at the initial stage to rapidly depolymerize surface pectin, followed by sustained expression of hemicellulases that ensured the complete removal of residual non-cellulosic materials. Quantitative real-time PCR (qRT–PCR) results showed strong concordance with the proteomic data, confirming that transcriptional regulation drives the dynamic enzymatic response. This integrative analysis, linking macroscopic morphological evolution with microscopic molecular changes, elucidates the intrinsic mechanism underlying efficient biological degumming by D. dadantii strain DCE-01 and provides valuable insights for the rational design of high-performance engineered bacteria for industrial fiber processing.