Fuente: PubMed "smart farming" Anim Nutr. 2026 Mar 6;25:433-448. doi: 10.1016/j.aninu.2025.11.010. eCollection 2026 Jun.ABSTRACTResveratrol has been shown to alleviate endoplasmic reticulum stress (ERS), lipid deposition and inflammation induced by high-fat diets in fish and mammals. However, whether dietary resveratrol supplementation in vegetable oil-based diets of fish can exert these beneficial effects remains unclear. A 56-d feeding trial was conducted to explore the impacts of resveratrol supplementation in a soybean oil-based diet (SO) on the growth and liver lipid deposition, ERS, inflammation and apoptosis in large yellow croaker (Larimichthys crocea). The fish (35.56 ± 0.26 g) were randomly distributed into 18 cages (50 fish/cage) corresponding to triplicate cages of the six diets, including a fish oil-based diet (FO), SO, and SO supplemented with 0.05% (SR0.05), 0.10% (SR0.1), 0.20% (SR0.2), and 0.40% (SR0.4) resveratrol, respectively. The results showed that the total replacement of fish oil by soybean oil significantly reduced final body weight (FBW) and percent weight gain (PWG) (P < 0.05), and significantly increased feed conversion ratio (FCR) and feed intake (FI) (P < 0.05). The SR0.1 group exhibited significantly higher PWG and lower FCR than the SO group (P < 0.05) and similar FBW and FI to the FO group (P > 0.05). The FO and SR0.1 groups shared similar liver crude lipid and triglyceride contents (P > 0.05), which were significantly lower than those of the SO groups (P < 0.05). Compared to the FO treatment, the livers of the SO groups showed severe vacuolation, increased size and number of lipid droplets, and swollen endoplasmic reticulum lumen. These changes were markedly alleviated in the SR0.1 group. Dietary soybean oil significantly increased the relative mRNA expression levels of ERS-related genes (grp78, xbp1, atf6α, atf4, and chop), pro-inflammatory cytokines (tnfα and il-1β) and pro-apoptotic genes (bax, caspase 3, and caspase 9), as well as the protein expression levels of ERS-related proteins (GRP78, XBP1, and ATF6) and phosphorylation levels of PERK, NF-κB p65 and JNK (P < 0.05). Meanwhile, dietary soybean oil decreased the relative mRNA expression levels of sirt1, anti-inflammatory cytokines (arg1 and il-10), anti-apoptotic gene (bcl-2), and the protein expression level of SIRT1 in the liver (P < 0.05). Resveratrol supplementation in the SO restored the expression of the above genes and proteins to some extent. In conclusion, supplementation with 0.10% resveratrol in the SO improved PWG and feed utilization, promoted SIRT1 expression and alleviated lipid deposition, ERS, inflammation and apoptosis in the liver of large yellow croaker.PMID:42004261 | PMC:PMC13089138 | DOI:10.1016/j.aninu.2025.11.010