Fecha de publicación: -- Fuente: PubMed "smart farming" J Nutr. 2026 Oct 26;156(12):101902. doi: 10.1016/j.tjnut.2026.101902. Online ahead of print.ABSTRACTBACKGROUND: Fatty liver syndrome (FLS) in laying hens is associated with gut dysbiosis.OBJECTIVES: This study aimed to explore the underlying mechanism by which gut dysbiosis contributes to the pathogenesis of FLS in laying hens.METHODS: In experiment 1, 68-wk-old laying hens that were healthy or had FLS (n = 10 birds/group) were selected on the basis of pathological changes in liver morphology. In experiment 2, 40-wk-old breeder hens (n = 16 birds/group) were given either a basal diet (CON) or CON supplemented with 1.5 kg/t organic acid blends (OA1 and OA2) for 12 wk. The production performance, gut barrier integrity, hepatic histopathology, gene expression, and gut microbiota were analyzed. The omics data were analyzed using bioinformatics tools, whereas the other data were analyzed using Student's t tests.RESULTS: Compared with healthy hens, hens with FLS exhibited increases (P < 0.05) in total cholesterol (TC) and triglyceride (TG) levels in both the serum and liver by 69.9% to 267.8%, and their serum lipopolysaccharide levels increased (P < 0.05) by 27.3%. Integrated omics analysis revealed that the abundance of the Rikenellaceae RC9 gut group increased (P < 0.05), whereas the pathways for histidine, glycine, serine, and threonine metabolism were inhibited in the livers of hens with FLS. Furthermore, dietary supplementation with OA1 and/or OA2 reduced (P < 0.05) the incidence of deformed eggs by 21.1% to 25.8% during weeks 7 to 12 and decreased (P < 0.05) liver TG and TC contents by 26.3% to 60.7% compared with the CON group. Meanwhile, OA1 and OA2 reduced (P < 0.05) serum lipopolysaccharide levels by 23.4% to 25.6%, whereas OA2 upregulated (P < 0.05) the expression of the cecal tight junction gene Claudin-1 by 111.6%.CONCLUSIONS: FLS progression in aged laying hens is primarily due to increased gut barrier impairment and bacterial translocation. OA supplementation improved gut barrier integrity and mitigated the occurrence of FLS in hens.PMID:42933648 | DOI:10.1016/j.tjnut.2026.101902