Fuente: Molecules - Revista científica (MDPI) Molecules, Vol. 31, Pages 1326: N-Methylated Nucleobases Crystal Structures and π-π Stacking Interactions
Molecules doi: 10.3390/molecules31081326
Authors:
Riccardo Cameli Manzo
Volodymyr Baran
Artem Shevchenko
Anastasia Sleptsova
Frank Hoffmann
Tomislav Stolar
Robert E. Dinnebier
Martin Etter

Solid-state studies evaluating intermolecular geometries in methylated nucleobases are not extensively explored. In the course of the present study, we have solved the crystal structures of 1-, 3- and 7-methylated adenines and guanines, including the monohydrate and sesquihydrate forms of 3-methyladenine and 3-methylguanine, respectively, by means of single-crystal X-ray diffraction and synchrotron/laboratory X-ray powder diffraction (XRPD). In situ high temperature XRPD experiments, coupled with differential thermal analysis/thermogravimetry (DTA/TG) measurements, allowed for monitoring crystallographic changes after water removal of N3-methylated compounds, and the discovery of a high temperature polymorph in the case of 3-methyladenine. Our findings indicate that H-bonding schemes describe ribbon planar motifs of molecules in the majority of cases, or linear double-bonded strands of molecules in a few cases. π-π stacking interactions were compared with existing findings of theoretical calculations and existing crystallographic data, showing how N-methylated purine bases follow the trend predicted by Hunter and Sanders, 1990. The present study provides the first systematic experimental insights into the solid state of the presented compounds.