Fecha de publicación: 20/01/2025 Fuente: Molecules - Revista científica (MDPI) Molecules, Vol. 30, Pages 423: Colorimetric Detection of Dopamine Based on Peroxidase-like Activity of β-CD Functionalized AuNPs
Molecules doi: 10.3390/molecules30020423
Authors:
Sara Anderson
Hamish Shepherd
Kiran Boggavarapu
Janak Paudyal

Catalytically active nanomaterials, or nanozymes, have gained significant attention as alternatives to natural enzymes due to their low cost, ease of preparation, and enhanced stability. Because of easy preparation, excellent biocompatibility, and unique optoelectronic properties, gold nanoparticles (AuNPs) have attracted increasing attention in many fields, including nanozymes. In this work, we demonstrated the applicability of beta-cyclodextrin functionalized gold nanoparticles (β-CD-AuNPs) as enzyme mimics for different substances, including TMB and DA. We found that β-CD-AuNPs can catalyze the H2O2-mediated oxidation of DA. The dopamine signal-off sensor was developed by taking advantage of the peroxidase-like activity of β-CD-AuNPs towards TMB and DA, where both 3,3′,5,5′-tetramethylbenzidine (TMB) and dopamine (DA) may compete for the binding sites with β-CD-AuNPs. As a result, the presence of dopamine can be detected even through the naked eye (up to the concentration of 3.75 µM) and using a spectrophotometer (up to the concentration of 1.0 µM) by monitoring the disappearance of the blue color of the oxidized form of TMB in the presence of dopamine. Furthermore, no obvious disappearance of color was observed at lower concentrations of interferences including ascorbic and uric acid. Given the versatility of cyclodextrin to host large numbers of analyte molecules, we envision that a similar principle can be applied for the detection of other analyte molecules of biological, medical, and environmental significance.