Fecha de publicación:
20/11/2020
Fuente: WIPO "organic farming"
A method of preparing an organic crop bio-stimulant is disclosed. The method comprising steps as chopping a fixed amount of fresh Lucerne plant material by a chopping technique; mixing a fixed amount of distilled water with the chopped Lucerne material in a conical flask; mixing a fixed amount of pectinase and cellulase enzyme with the Lucerne material and distilled water into said conical flask in order to prepare a mixture solution; incubating the mixture solution below ambient temperature for a fixed time period in order to properly digest said Lucerne plant material by pectinase and cellulase enzyme of the mixture solution; separating a liquid fraction of said incubated mixture solution from a solid fraction by a filter technique; and deactivating enzymes present in said separated liquid fraction by heat treating the liquid fraction at a fixed temperature for a fixed time period.