Fuente: WIPO "varroa" The invention discloses a method for measuring acute and chronic toxicity of a drug against varroa jacobsoni. The method comprises the following steps: gently spraying the to-be-measured liquid onto the surfaces of healthy bee larvae; transferring the bee larvae from the inside of a larva culture plate into a bee pupa culture cup with the liquid-infiltrated filter paper piece at the bottom; collecting the to-be-measured varroa jacobsoni; introducing a varroa jacobsoni into the bee pupa culture cups of each treatment group and control group, and sealing the cup rims of the bee pupa culture cups with ventilated transparent films to prevent the introduced varroa jacobsoni from escaping the bee pupa culture cups; putting the bee pupa culture cups into an incubator for culturing, wherein the temperature is 34.5 DEG C, and the relative humidity is 75%; counting the deaths of varroa jacobsoni 48h or 72h later, calculating the death rate and analyzing the acute toxicity of the drug against varroa jacobsoni; or continuously recording the survival conditions of the varroa jacobsoni every day, counting the survival time of each varroa jacobsoni, and analyzing the chronic toxicity of the drug against varroa jacobsoni. The method disclosed by the invention has the advantages of controllable experiment conditions, high survival rate of the varroa jacobsoni, long survival time and accurate and credible result.