Fuente: PubMed "bee pollen" J Insect Sci. 2026 Mar 2;26(2):ieag019. doi: 10.1093/jisesa/ieag019.ABSTRACTBumble bee microcolonies have been used to investigate a range of research questions including pesticide effects. As a toxicity test, the microcolony can be a tool to observe concentration-based endpoints and has the flexibility for delivering a test substance via sucrose solution, pollen provision, or both. However, there is no regulatory guideline available nor an academic standard experimental design for bumble bee microcolonies and test performance can be influenced by pollen source, pollen amount, and worker selection. Our objectives were to evaluate the impact of worker size and age, as well as pollen quantity and quality, on microcolony test performance of common eastern bumble bee (Bombus impatiens Cresson). A cumulative 5 g of pollen provision during the first 10 d of colony establishment resulted in a higher number of emerged males at the end of the test compared to 2 g. The number of emerged males was different depending on the source and nutritional composition of the pollen. Mixed sizes and ages of workers yielded good test performance and are recommended based upon our findings. These collective results improve test performance (ie maximize the number of produced drones, maintain worker survival, and enable consumption of sucrose solution and pollen provisions, the latter as potential vehicles of test items in a toxicity test) when conducting a bumble bee microcolony study. These findings enhance the reliability of bumble bee microcolony studies and provide essential insights for developing standardized testing protocols that can better assess the impacts of environmental stressors on pollinator health.PMID:41824760 | PMC:PMC12986759 | DOI:10.1093/jisesa/ieag019