The present invention relates to the field of biotechnology, and in particular to SNP markers as well as use and detection method thereof. The present invention employs whole genome sequencing to conduct whole genome sequencing on a large number of individuals of Apis mellifera sinisxinyuan, and obtains 21 pieces of SNP information specific in Apis mellifera sinisxinyuan, which become marker SNP sites for identifying Apis mellifera sinisxinyuan. The SNP sites provided by the present invention, which are specific in Apis mellifera sinisxinyuan, play important roles in the differentiation of western honey bee in China from the western honey bees in other regions, and in the adaptive evolution of western honey bee in China to the local environment.