Fecha de publicación: 18/11/2010 Fuente: WIPO "apiculture" The present invention relates to protection against honeybee mites, and method for the preparation of the active substance and the application of the active ingredient of the product in a preparation for protection against honeybee mite pests. The main point of the preparation that it contains vegetable oil, containing alkaloids with piperidine structure obtained from poison hemlock plant as active ingredient. The procedure of the invention relates to extracting alkaloids with piperidine structure from poison hemlock plant, when the portion of the plant above ground is harvested, its humidity content is decreased and suitably chopped, and the solvent extraction is carried out in two steps a) first at 85-90 bar pressure, and after dissolving the active substance at 280-310 bar pressure, or b) first at 85-90 bar pressure, then at 28-35 bar pressure, in both cases the temperature is kept at 25-35 °C, and the substance obtained is separated in two steps, first at 75-80 bar pressure, at 28-35 °C, then at 20 bar pressure, at 20-27 °C, the concentration of the active substance obtained as 76-78 w% solution is preferably adjusted to 45-50% active substance content with water dilution.