Fecha de publicación: 07/05/2014 Fuente: WIPO "royal jelly" The invention discloses an ultrafiltration membrane separation method for obtaining royal jelly major protein and active filtrate in royal jelly. The method comprises the following steps: weighing royal jelly, stirring and mixing uniformly with water at the temperature of 4 DEG C, regulating pH by using NaOH, regulating the ion strength by using NaCl, and removing particles by using a 0.2mu m microfiltration membrane; separating and removing macromolecular impurities by using an ultrafiltration membrane M1 with the molecular weight cutoff of 100kDa, separating and concentrating the royal jelly major protein in the M1 filtrate by using an ultrafiltration membrane M2 with the molecular weight cutoff of 49kDa, supplementing purified water when the royal jelly major protein is concentrated to be 1/3 volume, allowing a small molecule solution to pass through the M2, wherein the obtained cutoff solution is a purified and concentrated royal jelly major protein solution, and performing freeze drying to form the royal jelly major protein freeze-dried powder. The small molecule solution contains polysaccharides and 10-hydroxy-2-caproleic acid (10-HDA) and can be used for production of functional drinks. The royal jelly major proteins (MRJPs) are separated and concentrated by using an ultrafiltration device, the extraction rate of the MRJPs is 82.63 percent, and the royal jelly major protein is subjected to freeze-drying to form the freeze-dried powder with the protein content of more than 70 percent. Moreover, the filtrate is used for preparing drinks, and comprehensive utilization of the royal jelly is realized.