The invention relates to a royal jelly sucking device of an automatic royal jelly taking machine and belongs to the technical field of agricultural engineering. The royal jelly sucking device comprises a support mechanism, a royal jelly and larva separation tank, a centrifugal fan and royal jelly sucking heads and is characterized in that the support mechanism, the royal jelly and larva separationtank and the centrifugal fan are mounted on a frame, the royal jelly sucking heads are mounted on the support mechanism, the row space and head spacing of suckers at the ends of the royal jelly sucking heads are consistent with those of royal cells on a royal jelly producing bar, the suckers are hollow sucking pipes located above the royal jelly producing bar, main pipes are formed in the royal jelly sucking heads through the hollow pipelines, and the radial size of the heads of the suckers is smaller than the radial size of the roots of the suckers; the royal jelly outlets of the royal jellysucking heads are communicated with the royal jelly and larva separation tank through a royal jelly guide soft tube. The royal jelly sucking device has the advantages that the stack condition of royal jelly is guaranteed; a vibration screen combining with royal jelly sucking air flow blowing can effectively increase royal jelly and larva separation speed to achieve royal jelly and larva separation; high royal jelly taking efficiency and few residues are achieved.