Fecha de publicación: 30/11/2021 Fuente: WIPO "royal jelly" The invention discloses a royal jelly separation liquid fermentation product, a skin external preparation containing the royal jelly separation liquid fermentation product, and a preparation method and application. The preparation method of the royal jelly separation liquid fermentation product comprises the following steps that lactic acid bacteria are inoculated into a fermentation substrate, and fermentation culture and sterilization are carried out to obtain the royal jelly separation liquid fermentation product; and the fermentation substrate comprises a royal jelly separation liquid, and the royal jelly separation liquid is a material prepared by removing protein from fresh royal jelly. The royal jelly separation liquid fermentation has good antioxidant and anti-inflammatory effects, has the effect of promoting the growth of human skin fibroblasts, is high in safety, does not irritate the skin, is simple in preparation process and low in preparation cost, and can be widely applied to the field of external preparations for the skin.