Fecha de publicación: 29/03/2019 Fuente: WIPO "royal jelly" The invention provides a method for increasing yield of major proteins 2 and 3 in royal jelly by liquid chromatography purification. The method comprises steps as follows: royal jelly is dissolved inan extraction buffer solution, the mixture is mixed uniformly and centrifuged, a supernatant is taken, saturated ammonium sulfate is added to the supernatant for precipitation, a supernatant is takenafter centrifugation, filtering is performed by a filter membrane, and a sample buffer solution is obtained; the sample buffer solution is replaced with a buffer solution A with pH being 7.0 by an ultrafiltration centrifuge tube; a royal jelly sample is loaded to a pre-assembled weak cationic chromatographic column balanced by the buffer solution A; the column is subjected to gradient elution by amixed solution of the buffer solution A and a buffer solution B, the ratio of the buffer solution B is gradually increased from 0 to 100%, and major proteins 2 and 3 in the royal jelly are separatedand purified in sequence. According to the method for increasing the yield of the major proteins 2 and 3 in the royal jelly by liquid chromatography purification, abundance of the major proteins 2 and3 in the royal jelly is increased, yield of the major proteins 2 and 3 in the royal jelly is increased, repeatability is good, the yield is high, and the major proteins 2 and 3 in the royal jelly canbe produced on a large scale.