The present invention relates tothe composition as an intracanal medicament in endodontictreatment of permanent teeth. It particularly relates to gel composition as an intracanal medicament in endodontic treatment of permanent teeth. More specifically, it relates to propolis gel as an intracanal medicament in endodontic treatment of permanent teeth. The invention also includes the process for preparation of propolis gel as an intracanal medicament in endodontic treatment of permanent teeth. Propolis powder was procured and authenticated after which it was subjected to MIC and MBC procedures. 25% propolis gel was prepared according to the MBC values as(a) preparation of carboxymethylcellulose solution(b) addition of effective amount of propolis powder to above prepared carboxymethylcellulose solution and (c) then addition of other suitable excipients to the above mixture to form the gel composition A cytotoxicity test was done which concluded that propolis had a minimal cytotoxicity. In vitro tests were done which concluded that 25% propolis gel had a good antibacterial activity. In vivo test confirmed our in vivo results suggesting that propolis could be used as an intracanal medicament in endodontic treatment of permanent teeth.