A preparation method of wall broken bee pollen of Chuzhou chrysanthemum is characterized by comprising the following preparation steps: washing bee pollen of Chuzhou chrysanthemum with water, removing dust on the surface of the bee pollen of Chuzhou chrysanthemum, filtering and removing the water; adding the water-washed bee pollen of Chuzhou chrysanthemum into cyclodextrin and ingredients, mixing with water according to the ratio of 1:5-1:100, after treatment with a colloid mill, sieving with a 100 to 200 mesh sieve to obtain an even serous fluid of the bee pollen of Chuzhou chrysanthemum; after sterilization at pasteurization temperature of the even serous fluid of the bee pollen of Chuzhou chrysanthemum, using spray drying equipment with the air inlet temperature of 180-210 DEG C and the air outlet temperature of 80-100 DEG C for drying to obtain the wall broken bee pollen of Chuzhou chrysanthemum. The preparation method has the advantages of simple process and low cost, micro capsules of the cyclodextrin have a protective effect on the wall broken bee pollen of Chuzhou chrysanthemum, and the obtained wall broken bee pollen of Chuzhou chrysanthemum is easily miscible with water.