Fecha de publicación: 13/06/2017 Fuente: WIPO "apis cerana" The invention discloses a plant fragrance smell activator formula screening method based on apis cerana odorant binding protein. The method comprises the following process steps: 1) extracting working bee antenna total RNA (Ribonucleic Acid) of apis cerana; 2) designing an apis cerana odorant binding protein gene primer, and acquiring the full length of an apis cerana odorant binding protein gene through RT-PCR (Reverse Transcription-Polymerase Chain Reaction); 3) building a prokaryotic expression vector of the apis cerana odorant binding protein gene; 4) inducing recombinant expression of the apis cerana odorant binding protein through IPTG (isopropyl-beta-d-thiogalactoside), and performing purification through a Ni Sepharose FF affinity column; 5) acquiring a combined response spectrum of the apis cerana odorant binding protein and plant fragrance smell through a competitive fluorescent combination method, and determining the combined response spectrum as the plant fragrance smell activator suitable for the apis cerana when the dissociation constant KD is lower than 20mummol/L.