Fecha de publicación: 16/10/2020 Fuente: WIPO "apis cerana" The invention is suitable for the technical field of analysis of olfactory systems of bees, and provides an experimental method for in vivo and in vitro functional analysis of an olfactory receptor gene AcerOr1 of apis cerana cerana. An olfactory receptor AcerOr1 of apis cerana cerana is selected as a study object, and potential biological functions of the olfactory receptor AcerOr1 are explored in vivo and in vitro; subcellular location of an AcerOr1 protein structure is predicted to find that subcells of the AcerOr1 protein structure are located on a cell membrane; membrane topology structures are predicted to find that no calmodulin binding site area or olfactory ligand-gated channel is predicted in a sequence structure of the AcerOr1; an eukaryotic expression vector pIB/V5-AcerOr1 is successfully constructed and a stably transfected cell line is established; a Western blot result proves that the recombinant eukaryotic expression vector can be expressed in an insect cell Sf9; immunofluorescence shows that the recombinant eukaryotic expression vector is expressed on a cell membrane Sf9 and is consistent with the predicted result; and the AcerOr1 can form an ion channel on the cell membrane Sf9 together with endogenous Orco in Sf9, can form a heterodimer together with a traditional olfactory receptor, has the effect of the olfactory ligand-gated channel and can cause a changein Ca concentration after the cell is affected by an external stimulus, therefore, the effect of the AcerOr1 on the olfactory function of the apis cerana cerana can be analyzed according to experimental results, the experimental operation is convenient, and an effective theoretical basis is provided for the study of the olfactory system of the apis cerana cerana.2+>