The invention provides an artificial breeding method for queen Chinese honeybees, and belongs to the technical field of bee propagation. The method comprises the steps that male bees are bred by being fed on high-protein pollen powder feed as rewards; strong swarms are preferably selected as male and female parent swarms; large eggs are obtained by limiting egg laying; wax bowls and queen breeding frames are treated with honey water in which queen bee hormones are released; the bees are fed on specialized high-protein feed and feed with high microelement content for supplementary in the feeding period. According to the artificial breeding method, the queen bees with the large size and the high egg production capacity can be obtained, the queen breeding success rate is high, and swarms with strong colony population and high worker honeybee foraging capacity and swarm disease resistance can be obtained in subsequent Chinese honeybee breeding.