Fecha de publicación: 28/02/2020 Fuente: WIPO "apis mellifera" The invention provides a method for quantifying Apis mellifera honey MRJP3 through liquid chromatography-tandem mass spectrometry. Aiming at the Apis mellifera honey, characteristic peptide fragmentsof royal jelly major protein 3 (MRJP3) in the Apis mellifera honey are screened out, a quantitative method of liquid chromatography tandem mass spectrometry is developed, and the method comprises thesteps of screening the characteristic peptide fragments, making a standard curve, carrying out sample pretreatment, carrying out liquid chromatography separation, carrying out tandem mass spectrometrydetection, carrying out software quantification on the peptide fragments and the like. The method for quantifying the MRJP3 in the Apis mellifera honey, provided by the invention, has the advantagesof higher accuracy, precision and sensitivity and strong stability, and is suitable for accurately quantifying the MRJP3 in the Apis mellifera honey. The method can be used for assisting authenticityevaluation of Apis mellifera honey. In addition, the quantitative method has important practical significance in protecting rights and interests of honey consumers and maintaining healthy developmentof the bee product industry.