Fecha de publicación: 28/02/2020 Fuente: WIPO "apis mellifera" The invention provides a method for quantifying Apis mellifera honey MRJP2 through liquid chromatography-tandem mass spectrometry. Characteristic peptide fragments of Apis mellifera honey royal jellymajor protein 2 (MRJP2) are screened out, a quantitative method of liquid chromatography tandem mass spectrometry is developed, and the method comprises the steps of screening of the characteristic peptide fragments, making of a standard curve, performing sample pretreatment, performing liquid chromatography separation and tandem mass spectrometry detection, carrying out software quantification ofthe peptide fragments and the like. The method for quantifying the MRJP2 in the Apis mellifera honey, provided by the invention, has the advantages of higher accuracy, precision and sensitivity and strong stability, and is suitable for accurately quantifying the MRJP2 in the Apis mellifera honey. The method can be used for assisting authenticity evaluation of Apis mellifera honey. In addition, the quantitative method has important practical significance in protecting rights and interests of honey consumers and maintaining healthy development of the bee product industry.