Fecha de publicación: 02/10/2020 Fuente: WIPO "apis mellifera" The invention relates to the field of biotechnology and immunodetection, in particular to an anti-apis mellifera MRJP1 monoclonal antibody and a colloidal gold detection card. The invention provides ahybridoma cell strain 1F 61D 7 and a hybridoma cell strain 6F 124B 4 which stably secrete the anti-apis mellifera MRJP1 monoclonal antibody, the secreted antibody only recognizes apis mellifera MRJP1protein, does not recognize apis cerana MRJP1 protein, has the advantages of good specificity, high sensitivity and the like, and has no cross reaction with other family related proteins. The colloidal gold detection card and test strip of the apis mellifera MRJP1 are further developed from the antibody, the colloidal gold detection card and test strip have the advantages of high precision, smallbatch difference and the like, rapid detection of the apis mellifera MRJP1 in a bee product is achieved, and the colloidal gold detection card and test strip are particularly suitable for high-efficiency and large-batch detection of whether apis mellifera honey is mixed into Chinese honey (local honey) or not, and are particularly suitable for high-efficiency and large-batch detection of whetherapis mellifera honey is mixed into Chinese honey (local honey) or not