The present invention relates to a method for separating and purifying melittin (Mn), apamin (Ap), mast cell degranulation (MCD) peptide and other components present in bee venom (BV). The method allows the beneficial components of the venom to be retained in the purified BV substance and allergens to be eliminated, using ultrafiltration and silica gel chromatography. The combination of the two techniques eliminates allergenic and harmful components, such as the enzymes phospholipase A2 (PLA2) and hyaluronidase (HYA), and a purified substance (referred to as "PDA") is obtained from the venom. The invention also relates to an anti-ageing cosmetic product comprising the purified substance, wherein the cosmetic product is selected from a cosmetic serum.