Fuente: PubMed "apiculture" J Ethnopharmacol. 2026 Apr 17:121700. doi: 10.1016/j.jep.2026.121700. Online ahead of print.ABSTRACTETHNOPHARMACOLOGICAL RELEVANCE: Pulicaria dysenterica Gaertn. subsp. dysenterica has been traditionally used for gastrointestinal and inflammatory disorders; however, its phenolic stability during digestion and its anti-inflammatory efficacy have not been comprehensively investigated.AIM OF THE STUDY: This study aimed to evaluate the phenolic profile, antioxidant capacity, gastrointestinal stability, acute oral toxicity, and therapeutic efficacy of P. dysenterica subsp. dysenterica decoction extract.MATERIALS AND METHODS: Total phenolic content (TPC) and antioxidant activity (DPPH and CUPRAC assays) were assessed before digestion and after simulated gastric and intestinal digestion. Phenolic compounds were characterized using a validated UHPLC-HESI-Orbitrap®-HRMS2 method. Acute oral toxicity was evaluated according to OECD guideline 423. The therapeutic effects of undiluted extract (PDE-100) and a 50% dilution (PDE-50) were investigated in an experimentally induced hemorrhoid model by assessing rectal-anal coefficient (RAC), inflammatory index, and TNF-α levels in serum and rectal tissues.RESULTS: The undigested extract exhibited the highest TPC (11541.03 ± 17.34 mg GAE/g) and DPPH activity (616.22 ± 0.39 TE/g), whereas gastric digestion showed superior CUPRAC activity (1255.36 ± 16.14 TE/g). Among 78 screened phenolics, 28 were identified, with chlorogenic acid (8543.88 ± 38.04 μg/g, gastric phase) and caffeic acid (6412.00 ± 96.50 μg/g, intestinal phase) being predominant. No acute oral toxicity was observed up to 2000 mg/kg. PDE-100 significantly reduced RAC, inflammatory index, and TNF-α levels, while PDE-50 exhibited limited effects. TNF-α levels correlated positively with inflammation severity.CONCLUSION: P. dysenterica subsp. dysenterica is a safe, phenolic-rich plant with strong antioxidant and anti-inflammatory properties, supporting its potential as a natural therapeutic candidate for pharmaceutical and nutraceutical applications.PMID:42002117 | DOI:10.1016/j.jep.2026.121700