Fuente: PubMed "pollen" Plant Signal Behav. 2026 Dec 31;21(1):2670874. doi: 10.1080/15592324.2026.2670874. Epub 2026 May 29.ABSTRACTThe normal production of pollen and proper pollen-pistil interaction are critical for double fertilization in Arabidopsis. Previous studies have demonstrated that DHQS affects female gametophyte development and pollen tube funicular guidance during fertilization. However, whether DHQS influences pollen development and pollen-pistil interaction remains unknown. In this study, we analyzed pollen and sperm cell morphology in dhqs-1/+ mutant. Results showed that DHQS does not affect sperm cell formation but significantly reduces pollen grain size. Pollen germination assays revealed no impairment in dhqs-1 pollen germination. However, semi-in vitro experiments demonstrated that dhqs-1 pollen tubes exhibited delayed growth through stigmas compared to wild-type. Notably, supplementation of shikimic acid in the medium significantly alleviated the growth inhibition of dhqs-1 pollen tubes in wild-type stylar tissue, underscoring the essential role of shikimic acid in mediating pollen-pistil interaction. These findings suggest that DHQS regulates pollen-pistil interaction through the shikimic acid pathway, providing novel insights into the molecular mechanisms underlying fertilization in plants.PMID:42216477 | DOI:10.1080/15592324.2026.2670874