Fecha de publicación: 03/12/2024 Fuente: PubMed "pollen" PLoS One. 2024 Dec 3;19(12):e0313954. doi: 10.1371/journal.pone.0313954. eCollection 2024.ABSTRACTChina possesses a copious and geographically diverse reservoir of hazel (Corylus spp.) resources, albeit scholarly endeavors focusing on the selection and domestication of indigenous wild hazelnut strains remain scant. To develop and utilize high-quality wild hazelnut resources, this study selected eight populations of wild hazelnuts from seven different provenances in Heilongjiang Province, China. Natural hybrid seeds of eight populations were sown in the Chohai Forest Farm in Longjiang County, Heilongjiang Province, in 2018. In April 2020, two-year-old seedlings were used to establish a provenance trial forest, thereby initiating the provenance trial. Growth parameters were measured using electronic calipers, and pollen characteristics were observed under an electron microscope. The trials meticulously explored the trees' flowering biology, growth parameters, and the economic attributes of the produced nuts. Principal component analysis was employed to comprehensively assess differences among the wild hazelnut populations from various provenances, aiming to identify superior wild hazelnut provenances. The results unveiled significant geographical variations among the tested provenances across several facets: the flowering period of male blossoms extended from April 2nd to April 19th, while female flowering occurred within the timeframe of April 2nd to April 22nd. Moreover, pollen grain morphology demonstrated variability, with the polar axis ranging from 14.02 to 17.09 micrometers, the equatorial axis spanning 16.02 to 18.75 micrometers, and the ratio of polar to equatorial axes fluctuating between 0.88 and 0.92. Through correlation analyses, nut length emerged as a pivotal determinant significantly influencing both kernel weight and the hundred-grain weight. A principal component analysis (PCA) further consolidated these findings, selecting the Nehe-originated variegated Corylus heterophylla as the superior provenance based on a comprehensive evaluation of its combined traits. This study constitutes a seminal contribution to the hazelnut breeding endeavor in China, establishing a robust foundation for informed cultivation strategies geared towards optimizing both the yield and the quality of hazelnut resources, thereby advancing the understanding and exploitation of China's wild hazelnut biodiversity.PMID:39625918 | DOI:10.1371/journal.pone.0313954